cellsens image deconvolution software Search Results


deconvolution  (Evident Corporation)
99
Evident Corporation deconvolution
Deconvolution, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellsens  (Evident Corporation)
99
Evident Corporation cellsens
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
cellsens - by Bioz Stars, 2026-04
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iterative deconvolution  (Evident Corporation)
99
Evident Corporation iterative deconvolution
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Iterative Deconvolution, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iterative deconvolution - by Bioz Stars, 2026-04
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cellsens deconvolution laser scanning confocal advanced maximum likelihood  (Evident Corporation)
90
Evident Corporation cellsens deconvolution laser scanning confocal advanced maximum likelihood
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens Deconvolution Laser Scanning Confocal Advanced Maximum Likelihood, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellsens deconvolution laser scanning confocal advanced maximum likelihood - by Bioz Stars, 2026-04
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cellsens software  (Evident Corporation)
99
Evident Corporation cellsens software
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
cellsens software - by Bioz Stars, 2026-04
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cellsens dimension 1.11 2d deconvolution algorithm software  (Evident Corporation)
90
Evident Corporation cellsens dimension 1.11 2d deconvolution algorithm software
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens Dimension 1.11 2d Deconvolution Algorithm Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellsens dimension 1.11 2d deconvolution algorithm software/product/Evident Corporation
Average 90 stars, based on 1 article reviews
cellsens dimension 1.11 2d deconvolution algorithm software - by Bioz Stars, 2026-04
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cell r software  (Evident Corporation)
99
Evident Corporation cell r software
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cell R Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
cell r software - by Bioz Stars, 2026-04
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deconvolution software cellsens  (Evident Corporation)
99
Evident Corporation deconvolution software cellsens
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Deconvolution Software Cellsens, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deconvolution software cellsens - by Bioz Stars, 2026-04
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cellsens dimension module  (Evident Corporation)
90
Evident Corporation cellsens dimension module
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens Dimension Module, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellsens advmle deconvolution algorithm  (Evident Corporation)
99
Evident Corporation cellsens advmle deconvolution algorithm
( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus <t>cellSens</t> deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015
Cellsens Advmle Deconvolution Algorithm, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus cellSens deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015

Journal: eLife

Article Title: AMP-activated protein kinase fortifies epithelial tight junctions during energetic stress via its effector GIV/Girdin

doi: 10.7554/eLife.20795

Figure Lengend Snippet: ( A ) Subconfluent monolayers of MDCK cells grown on cover slips were fixed and stained for α-Tubulin (green), pS245-GIV (red) and DAPI (blue; nuclei) and analyzed by confocal microscopy. Image A shows an xy-plane maximum projection of a deconvolved stack of images, acquired at 20-nm z-intervals using a Olympus cellSens deconvolution (constrained iterative) and restoration system (Olympus FV3000) and a Apochromat 60XOSC2, NA 1.4 objective. The total thickness of the image stack is 4.8 mm. Two regions of interest (ROI), 1 and 2, are indicated with white boxes. ( B–D ) 3D reconstruction of each ROI shows the close proximity of pS245-GIV (red) to connecting microtubules (green) running along the cell-cell junctions (arrowheads). Little or no pS245-GIV staining (red pixels) is seen on microtubules at the 'free' cell border in panel D (arrows). ( E – H ) Maximum projected ROI‘s 1 and 2 are magnified in E and G , respectively. Individual Z-stacks of each ROI is shown in . Single Z-stacks of ROI‘s 1 and 2 are displayed in F and H , respectively. The insets in panels F and H show red, green and merged channels of respective figures. The white line indicates the pixels used for generating the RGB profile plots shown in I and J . RGB profiles show that pS245-GIV (red pixels) often co-localizes either completely with microtubule tracks (green pixels; I ) or lay between two microtubule tracks ( J ) at the cell-cell junctions. DOI: http://dx.doi.org/10.7554/eLife.20795.015

Article Snippet: Images were processed using the 3D deconvolution and 3D reconstructions tools of the Olympus cellSens (version Dimension Desktop 1.15) software.

Techniques: Staining, Confocal Microscopy